The new mastercycler x50 is the elegant synthesis of speed, flexibility and pcr optimization for research pcr applications and standardization for routine pcr applications such as food testing etc. Learn more about thermal cyclers, mastercycler ep realplex. Eppendorf mastercycler ep gradient thermal cycler 96 well. Cellsdirect onestep qrtpcr kit with rox, for abi 7000, 7300, 7700, and 7900ht instruments.
Reliance onestep multiplex supermix is an ideal solution for multiplexed rtqpcr, especially for pathogenviral detection and high throughput screening applications. Mit dem mastercycler nexus x2 konnen sie zwei vollig unabhangige protokolle gleichzeitig ausfuhren. I havent used any of the cyclers you suggest, but ive been using eppendorfs mastercycler ep realplex for realtime pcr and i find it extremely reliable reverse transcription done with. Eppendorf 950010045 mastercycler ep gradient s thermal. The mastecycler pro is unparalleled in its ability to fulfill requirements. The thermoblock, the inner side of the heated lid and micro test tubes pcr. Amplify your performance to ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. Mastercycler nexus cyclers, pcr eppendorf south pacific. Mastercycler x50 cycler, pcr eppendorf deutschland. This flexibility allows pcr users to select the consumables that best fit their assay. Eppendorf mastercycler ep realplex realtime pcr system. One approach to assay optimization is to determine the optimum annealing temperature t a of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures. Find online auctions and classified ads for eppendorf products and more on the labx marketplace. This allows two pcr users in the lab to start and end their pcr reactions whenever they want.
Its compatibility to a wide range of predefined consumables also allows established procedures to be maintained. Combine any mix of mastercycler pro gradient s, mastercycler pro gradient, and mastercycler pro 384 to a network of up to 30 units and control the entire network with one computer. In our laboratory, we use it routinely for quantitative mrna expression studies, verification of microarray results and pathogen mycoplasma detection in eukaryotic systems. The eppendorf mastercycler family offers ultimate reliability in pcr thermal cyclers. If you need a ultrafast pcr between 515 minutes for most 30 cycle programs machine. The thermoblock, the inner side of the heated lid and micro test tubes pcr plates can reach temperatures. We enable science by offering product choice, services, process excellence and our people make it happen. Development and interlaboratory validation of unlabeled.
Eppendorf mastercycler ep realplex instrument setup instructions for rt2 profiler pcr arrays. It is a compact, easy to use and flexible platform. Control of this pcr system is easy thanks to the cycle manager pro software, that gives you a high degree of flexibility, and guides users with a easy to understand graphic interface. The mastercycler ep realplex features the latest technology, united with good design and a quality finish.
Mastercycler ep realplex from eppendorf biocompare product. The mastercycler nexus can accommodate 96well pcr plates, 0. This pcr system uses a control panel to manage reactions, and can be operated through the key pad or with use of a mouse to easily edit and store up to 700 protocols. The eppendorf mastercycler ep gradient thermal cyclers are known for their fast heating and cooling, with speedy gradient pcr cycling for the best in optimized reaction conditions. The epmotion 5070s pipetting technology is based on the classic eppendorf pistonstroke pipettes. Hence, the time it takes to complete an assay is decreased. The company operates in more than 30 countries and delivers an extensive portfolio of products and services. In addition, eppendorf offers a temperature validation. Mastercycler ep realplex from eppendorf selectscience. Abi 7500 applied biosystems, foster city, ca and mastercycler ep realplex eppendorf, hamburg, germany were used in shanghai. Our mastercycler pro has a special lid to avoid evaporation and superior block homogeneity to achieve consistent results, despite the well being used. Mastercycler x50 cyclers, pcr eppendorf international.
Realtime pcr is the simultaneous amplification and detectionquantification of nucleic acids using pcr. Primer optimization using temperature gradient protocol. The eppendorf mastercycler and mastercycler gradient are pcr devices for all molecular biological and biochemical laboratories in the. Most differences are obviously in the application software, especially in the way of. Two totally independent protocols can be run on the two blocks. Shop online for a wide selection of eppendorf mastercycler pro pcr system proprietary tect technology reduces evaporation to a minimum, improving reproducibility.
Browse ads and contact the seller directly or request a quote for more details. This can be achieved if a qpcr instrument with a temperature gradient block is available. Used eppendorf mastercycler ep realplex 2 realtime pcr. Ive been using more eppendorfs ep mastercycler gradient and i will be getting. Peltier elements control the temperature of the silver coated block with extremely fast heating and cooling rates. For completion, the following features concerning operation of the mastercycler ep realplex are not accessible to restricted users. Up to 30 units of any block format combination can be controlled with one computer. Temperature control is carried out with peltier elements, which enable extremely rapid changes in temperature of up to 3 c per second. Melting curve analysis was performed with the default settings on the abi 7500 system sds 1. The eppendorf mastercycler ep gradient s is a thermal cycler in 96well format. The mastercycler nexus x2 is eppendorfs solution for busy pcr labs. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. Testing equipment 11 accessory 1 calibration 2 calorimeter 2 colorimeter 0. Use the realtime pcr master mix selection tool to find optimized master.
The mastercycler pro is optimized for high throughput capabilities. Mastercycler nexus x2 cyclers, pcr eppendorf india. Effects of plate position, plate type and sealing systems on real. Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and research. We enable science by offering product choice, services, process excellence and our people make. This is a critical software update as ip addressing issues may lead to board failure, requiring board replacement. Mastercycler ep realplex software setup for the quantifast probe. Available realtime rtpcr cycler and kinetic pcr machines. Mastercycler nexus 360view mastercyclernexusgradient the mastercycler nexus can accommodate 96well pcr plates, 0. Mastercycler services from eppendorf selectscience. Uber unseren besonders intuitiven touchscreen sind all diese vorteile stets verfugbar. Eppendorf mastercycler ep realplex instrument setup instructions for. With less than 40 db a noise emission, the nexus is whisper quiet, while also being energy efficient.
Aug 20, 2009 mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. Mastercycler ep realplex realtime pcr system eppendorf. Reliance onestep multiplex supermix is designed to efficiently detect both rna and dna targets in the same reaction. The eppendorf mastercycler ep 384 thermal cycler system uses an aluminum block, which is a great conductor of heat and will heat uniformly throughout. Features and benefits sensitive, reproducible multiplexing of up to 5 targets get the most out of your samples while reducing costs. Mastercycler nexus x2 cycler, pcr eppendorf deutschland. A standard 40 cycle taqman assay can be run in approximately 80 minutes, including data analysis and report generation.
The advanced cyclemanager pro software offers a clientserver architecture, that enables one to check the status of the pcr from any computer in your local network. Data were analyzed using eppendorf mastercycler ep realplex 2. Temperature control is carried out with peltier elements, which enable extremely rapid changes in. The advanced cyclemanager pro software offers a clientserver architecture, that enables one to check the status of the pcr from any computer in. Sample processing solutions for laboratorydeveloped tests. T a of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures. Mar 20, 2008 the eppendorf mastercycler realplex 2 is a small, robustly designed instrument for realtime pcr. Connection of mastercycler ep realplex to a computer. The 96 well aluminium block can accomodate several standard vessels types from 0. In a normal working day in our laboratory, the realplex 2 can be run four to five times, depending upon assay type. Browse ads and contact the seller directly or request. Enjoy peace of mind with eppendorf performance plans and services for your mastercycler pro, mastercycler ep and mastercycler family thermal cyclers. This eppendorf cyclemanager pro software allows control of up to 30 mastercycler pro with one computer. Five different colors can help organize your workflow.
The mastercycler nexus pcr cycler provides scientists with flexibility in many forms. Ive been using the veriti thermal cycler even for gradients tests. Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and resear. A minimal number of moving parts in the optical detection module minimizes the vulnerability of the overall system and promotes consistently high sensitivity in your realtime pcr experiments. Eppendorfs mastercycler ep realplex is an instrument that exceeds the demand for rapid, accurate and reliable results. Mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. Modified slip cover light transmissive cover for optical openings in heated platen assembly, us patent pending. Mastercycler ep realplex software setup for the quantifast. Most differences are obviously in the application software, especially in the way of data analysis and how.
Reliance onestep multiplex supermix, 1,000 x 20 l rxns, 5. As our channel brand, vwr offers an integrated, seamless purchasing experience that is optimized for the way our. Mastercycler ep realplex realtime pcr solutions eppendorf has developed a realtime pcr system that meets the requirements of the latest quantitative pcr applications. Configuration of the database tool is only accessible for an administrator. The thermoblock, the inner side of the heated lid and micro test tubes pcr plates can. Whether samples are tested for both rna and dna viruses at the same time or are run with internal controls, reliance onestep multiplex supermix. Cyclemanager pro software benefit from a clientserver architecture and full software functionality. Eppendorf cyclemanager pro software life sciences, life. Mastercycler mastercycler gradient operating manual. The machine features, like some other mastercyler devices from eppendorf, a special 96well heating block, which enables rapid thermal cycling. The eppendorf mastercycler pro offers ultimate flexibility for your pcr applications. Connection of several mastercycler ep to one control panel. Mastercycler nexus cyclers, pcr eppendorf international.
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